BIACORE Instrumentation

 

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The facility supports the high performance BIACORE 3000. and provides training in the operation of the 3000 .  Training consists of a 1-day self-guided tutorial, augmented by practical hands on approach using an investigators sample material.  Prior to performing the hands on tutorial, it is recommended to read the Technology and SPR notes at Biacore’s web site. The facility has a copy of BIAsimulation that you may install on your computer.  This program allows one to perform dry run experiments to verify experimental data, save time on materials and time during experimental design and to use as a learning tool for BIA analysis.  Contact facility personnel to use BIAsimulation or to answer questions (FAQ).  A variety of CHIPs and chemistries are available to which a molecule of interest is attached, thus lending a certain degree of mobility to the bound molecule (ligand). The partner molecule (analyte) is then passed over this prepared surface under flow, and any interaction between partners is recorded as a function of time in resonance units (RU) -- these are arbitrary units that reflect the changes in refractive index (a function of mass) at the sensor surface that occur as a result of the binding of solution-phase analyte to (or its release from) surface-bound ligand.